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bismuth citrate, immune homeostasis, laboratory animals

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HrushkaО. I., Turkina, V. A., & Kuzminov, B. P. (2019). THE IMMUNOMODELING ACTIVITY OF BISMUTH CITRATE IN EXPERIMENT 1-CSTL AND LABORATORY OF INDUSTRIAL TOXICOLOGY. Clinical and Preventive Medicine, (3-4), 134-139.


The aim of the study. To investigate in experimental conditions the effect of bismuth citrate on the immune system of laboratory animals.

Material and methods. One-time sensitization of tadpoles (subcutaneously into the ear) was performed according to the method of O.G. Alekseeva, A.I. Petkevich. The degree of sensitization was established after skin tests. Determined the leucocyte blood formula, T and B lymphocytes in peripheral blood by the rosette method, immunoglobulins of class A, M, G in serum - by the method of radial immunodiffusion of globulines in agar Difco, circulating and extracellular complexes detection of the response of blood cells to the allergen "in vitro" - by the reaction of specific leukocyte lysis.

Results. Bismuth citrate causes impaired immunological homeostasis in experimental animals. Indicators of nonspecific cellular component of the immune system have changed significantly, characterizing immunocomplex pathology. A decrease in the T-helper subpopulation was observed, indicating a significant immunomodulatory ability of bismuth citrate. At the same time, there was a significant increase in cells capable of rosette formation, which suggests that bismuth citrate is capable of provoking sensitization of the body. In the humoral immunity link, significant differences in the content of circulating immune complexes in sensitized and control animals were observed. Immunoglobulin levels were unchanged relative to control. The analysis of the sensitizing effect indicates that the most probable result of sensitization of the organism in this mode of receipt of bismuth citrate is the formation of type III hypersensitivity.

Conclusions. Bismuth citrate under experimental conditions causes changes in the immune system of experimental animals, which are characteristic of the allergenic response of the organism to the complement-dependent type.
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